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Inorganic nitrogen enters the RAS mainly as an event of the feeding regime. Feed gives rise to feces and gill excretes from the fish, which releases urea, ammonia and ammonium as end products. In turn, the uneaten feed and fish feces nourish the heterotrophic bacteria load that releases ammonia as the end product, which is due toammonification of the organic nitrogen. Nitrogen compounds can be toxic to aquatic animals such as farmed fish, and can eventually limit the cultivation if the nitrogen
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